Ana Ivanovic said she was left shaken when, during her second round win over Anastasija Sevastova on Thursday morning, she heard a spectator crashing down a set of stairs.
The match was delayed for more than 25 minutes when a woman in the crowd at Rod Laver Arena fell at 11.35am.
The incident comes after Australian Bernard Tomic was left rattled when a tennis fan fell ill during his win over Denis Istomin on Tuesday night.
Tennis officials at the Australian Open said a health professional in the crowd went immediately to help the woman, first aid officers working at the event followed and she was eventually carried away on a stretcher.
Ivanovic was on serve leading 4-3 when play was halted, leaving the players to watch on.
Ivanovic and Sevastova were seen alternately sitting on the sidelines and pacing the court as the drama unfolded, before they briefly left the court.
They were allowed a warm-up hit before resuming the game and Ivanovic, ranked 23 in the world, went on to win the match 6-3, 6-3.
In the post-match press conference, Ivanovic said she immediately stopped play when she heard the woman fall.
“I just hope the lady was fine,” she said.
“It was actually good that we had to wait a little bit because I was really shaking, because I could imagine and it was not so nice.
“Then they told us she was bleeding, so, yeah, I hope she’s well.”
On Tuesday Tomic said he feared for a spectator’s life after a woman collapsed during his match and was taken from Hisense Arena on a stretcher.
Fellow spectators had on that occasion called for an EpiPen, which was thrown from one of the upper levels of the stadium.
Tomic was relieved to hear the ill woman had recovered.